A Review Of flow cytometers
A Review Of flow cytometers
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Information-enriched characterization of mobile populations. Morphological information and facts from photographs can insert to the richness of flow cytometry details. Such as, the figure exhibits an otherwise regular apoptosis assay utilizing Annexin V and PI, adding mobile imaging to characterize cells in Each individual inhabitants to reveal morphologically distinct functions. These insights couldn't have been attained from multiplex staining by itself.
As samples are acquired over the Attune CytPix Flow Cytometer, the superior-pace brightfield camera captures and retailers pictures of detected situations in a charge around six,000 photos for every next, based on the flow rate and impression measurement. For larger flexibility, Attune Cytometric Software permits you to alter the picture capture frequency as essential and select a specific gate for image seize, if necessary.
Incorporate acoustic concentrating engineering with higher-parameter Examination for adaptable spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing by using a large-speed brightfield digicam for simultaneous superior throughput flow cytometry and superior resolution brightfield imaging.
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Right here, a highly skilled consumer has gated singlets confidently. Right after assessing the handbook singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate is made up of over four% aggregates.
Pair your Attune flow cytometer with the autosampler for amplified workflow performance, and add robotic integration for max throughput.
Consistent image top quality even at substantial throughput Acoustic concentrating in addition to a higher-velocity digital camera Mix to image these T cells persistently at minimal or higher flow premiums. Quickly change concentrate and digital camera options to satisfy experimental requirements.
Early signs of this contamination had at first been dismissed as debris. Backgating to discover new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also means that you can use morphological features to discover exciting subpopulations that might not be evident from flow cytometry facts on your own.
Perhaps most significantly, these situations incorporate cells of Evidently diverse phenotypes which could lead to incorrect conclusions pertaining to double beneficial situations (especially in unusual populations).
Seem waves empower precise mobile alignment, causing Excellent details high quality and ~10X speedier sample flow rates.
Application note: Automated image Evaluation lowers person-to-person variability in flow cytometry gating strategies
Crafted-in dimensionality reduction tools improved resolution into populations. Fluorescently gated populations again-gated on to UMAP plot to verify expected population resolution in lysed total human blood. User can perspective activities from each cluster for Visible affirmation of cell phenotypes.
Flexible payment approach from Agilent Economical Answers let you receive the vital instrumentation you may need for quick project, or to increase your lab.
At the heart of every Attune Flow Cytometer lies our groundbreaking acoustic concentrating engineering, which utilizes seem waves to meticulously align cells inside the flow cell. This progressive tactic surpasses common hydrodynamic techniques, leading to speedier processing speeds, Excellent info good quality, and elevated sample throughput.
The Attune Xenith cytometer is really a large-parameter spectral analyzer, offering both equally spectral unmixing and standard flow capabilities for extensive and get more info functional mobile analysis.
The Attune CytPix Flow Cytometer will allow people to visualize samples for the duration of acquisition, confirm the precision of gating methods, and support success with reputable documentation for publication needs.
What seemed to be a homogeneous inhabitants is definitely extra numerous—an insight that ought to be deemed when interpreting results.